Always in every prayer. Connect the words in this manner: "Always presenting prayer for you all in every prayer of mine." For as he had said before, that the remembrance of them was an occasion of joy to him, so he now subjoins, that they come into his mind as often as he prays. He afterwards adds, that it is with joy that he presents prayer in their behalf. Joy refers to the past; prayer to the future. For he rejoiced in their auspicious beginnings, and was desirous of their perfection. Thus it becomes us always to rejoice in the blessings received from God in such a manner, as to remember to ask from him those things that we are still in need of.

Always - There is much emphasis in the expressions which are used here. Paul labors to show them that he never forgot them; that he always remembered them in his prayers.
In every prayer of mine - This was a proof of particular and special affection, that while there were so many objects demanding his prayers, and so many other churches which he had founded, he never forgot them. The person or object that we remember in every prayer must be very dear to the heart.
For you all - Not for the church in general, but for the individual members. "He industriously repeats the word 'all,' that he might show that he loved them all equally well, and that he might the more successfully excite them to the manifestation of the same love and benevolence" - Wetstein.
Making request with joy - With joy at your consistent walk and benevolent lives - mingling thanksgiving with my prayers in view of your holy walk.

Always in every prayer - I pray often for you, and have great pleasure in doing it, seeing what God has already wrought among you.

Always in every prayer of mine for you all,.... The apostle was a praying believer, and a praying minister: notwithstanding all his gifts, and graces, and high attainments, he was not above the work and duty of prayer, and in which he was sensible he stood in need of the assistance and direction of the Spirit of God, As soon as he was converted he prayed, and continued to do so without ceasing, as he himself directs; he was constant and assiduous at the throne of grace, and was concerned for others, as well as himself, for all the churches, and for this church, and all the saints in it,
Making request with joy; for what God had done for them, and continued with them. Requests are to be made known to God with thanksgiving. When we request a favour of him, it becomes us to return thanks for what we have received from him. Thanksgiving is a branch of prayer; as we have always mercies to ask for, we have always mercies to be thankful for.

making request--Translate, "making my request."
for you all--The frequent repetition in this Epistle of "all" with "you," marks that Paul desires to declare his love for all alike, and will not recognize any divisions among them.
with joy--the characteristic feature in this Epistle, as love is in that to the Ephesians (compare Philippians 1:18; Philippians 2:2, Philippians 2:19, Philippians 2:28; Philippians 3:1; Philippians 4:1, Philippians 4:4). Love and joy are the two first-fruits of the Spirit. Joy gives especial animation to prayers. It marked his high opinion of them, that there was almost everything in them to give him joy, and almost nothing to give him pain.

With joy - After the epistle to the Ephesians, wherein love reigns, follows this, wherein there is perpetual mention of joy. "The fruit of the Spirit is love, joy." And joy peculiarly enlivens prayer. The sum of the whole epistle is, I rejoice. Rejoice ye.