On this account the Father loveth me. There is, indeed, another and a higher reason why the Father loveth the Son; for it was not in vain that a voice was heard from heaven, This is my beloved Son, in whom the good-pleasure of God dwells, (Matthew 3:17; 17:5.) But as he was made man on our account, and as the Father delighted in him, in order that he might reconcile us to himself, we need not wonder if he declares it to be the reason why the Father loveth him, that our salvation is dearer to him than his own life. This is a wonderful commendation of the goodness of God to us, and ought justly to arouse our whole souls into rapturous admiration, that not only does God extend to us the love which is due to the only-begotten Son, but he refers it to us as the final cause. And indeed there was no necessity that Christ should take upon him our flesh, in which he was beloved, but that it might be the pledge of the mercy of his Father in redeeming us. That I may take it again. As the disciples might be deeply grieved on account of what they had heard about the death of Christ, and as their faith might even be greatly shaken, he comforts them by the hope of his resurrection, which would speedily take place; as if he said, that he would not die on the condition of being swallowed up by death, but in order that he might soon rise again as a conqueror. And even at the present day, we ought to contemplate the death of Christ, so as to remember, at the same time, the glory of his resurrection. Thus, we know that he is life, because, in his contest with death, he obtained a splendid victory, and achieved a noble triumph.

I lay down my life - I give myself to die for my people, in Jewish and pagan lands. I offer myself a sacrifice to show the willingness of my Father to save them; to provide an atonement, and thus to open the way for their salvation. This proves that the salvation of man was an object dear to God, and that it was a source of special gratification to him that his Son was willing to lay down his life to accomplish his great purposes of benevolence.
That I might take it again - Be raised up from the dead, and glorified, and still carry on the work of redemption. See this same sentiment sublimely expressed in Philippians 2:5-11.

Therefore doth my Father love me - As I shall be shortly crucified by you, do not imagine that I am abandoned by my heavenly Father, and therefore fall thus into your hands. The Father loveth me particularly on this account, because I am going to lay down my life for the life of the world. Again, do not suppose that I shall be put to death by your rulers, because I have not strength to resist them. I Lay Down my life voluntarily and cheerfully; no one can take it away from me, see John 10:18; and I shall give you the fullest proof of my supreme power by raising, in three days, that very crucified, wounded body from the grave.

(5) Therefore doth my Father love me, because (g) I lay down my life, that I might take it again.
(5) Christ is by the decree of the Father the only true shepherd of the true Church, for he willingly gave his life for his sheep, and by his own power rose again to life.
(g) He uses the present tense because Christ's whole life was as it were a perpetual death.

Therefore doth my Father love me,.... Christ was the object of his Father's love from all eternity, and was loved by him on various accounts; first and chiefly, as his own Son, of the same nature with him, equal to him; and also as Mediator, engaging for, and on the behalf of his chosen people; and likewise as he was clothed with their nature, and even in his state of humiliation; and not only as subject to his ordinances, and obedient to his will, and doing what was pleasing in his sight, but likewise as suffering in their room and stead, and he loved him on this account; the bruising of him was a pleasure to him, not for the sake of that itself, but because hereby his counsels and decrees were accomplished, his covenant fulfilled, and the salvation of his people obtained: hence it follows here,
because I lay down my life; that is, for the sheep; to ransom them from sin and Satan, the law, its curse and condemnation, and from death and hell, wrath, ruin and destruction: and the laying down his life on this account, was not only well pleasing to his Father, but likewise was done, with the following view; or at least this was the event of it,
that I might take it again; as he did, by raising himself from the dead, by which he was declared to be the Son of God; and to have made full satisfaction to divine justice, for the sins of his people, and therefore rose again for their, justification; and to be the victorious conqueror over death, having now abolished it, and having in his hands the keys of it, the power over that, and the grave: and which life he took up again, by his divine power, and as the surety of his people, to use it for their good; by ascending to his God and theirs, entering into heaven as their forerunner, appearing in the presence of God for them, as their advocate, and ever living to make intercession for them.

Therefore doth my Father love me, because I lay down my life, &c.--As the highest act of the Son's love to the Father was the laying down of His life for the sheep at His "commandment," so the Father's love to Him as His incarnate Son reaches its consummation, and finds its highest justification, in that sublimest and most affecting of all acts.
that I might take it again--His resurrection-life being indispensable to the accomplishment of the fruit of His death.

I lay down my life that I may take it again - I cheerfully die to expiate the sins of men, to the end I may rise again for their justification.